Ectopic expression of TAL-1 increases resistance to TNF[alpha]-induced apoptosis in Jurkat cells via changes in the NF-kB signaling pathway

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Authors
Lucas, Bethany R.
Advisor
Olesen, James B.
Issue Date
2011-05-07
Keyword
Degree
Thesis (M.S.)
Department
Department of Biology
Other Identifiers
Abstract

TAL-1, ectopically expressed in 60% of T-cell acute lymphoblastic leukemia (T-ALL) patients, may contribute to poor chemotherapy response. This research sought to determine if TAL-1 influences expression of proteins involved in the NF-kB signaling pathway and thus, resistance to cell death. NF-kB, IKKy, and TRAF-2 expression levels were found to be TAL-1 dependent. Cell death levels were higher in staurosporine-treated cells compared to tumor necrosis factor a-treated or dual-treated cells. TAL-1, NF-kB, IKKy, and TRAF-2 expression levels were elevated in tumor necrosis factor a-treated cells and reduced in staurosporine-treated or dual treated cells compared to untreated cells. These results suggest TAL-1 influences expression of proteins involved in the NF-kB signaling pathway, thus inducing an anti-apoptotic response in the cell.

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