Characterization of prodiginine compounds produced by a Vibrio species isolated from salt flat sediment along the Florida Gulf Coast

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Authors
Morgan, Stephanie
Thomas, Matthew J.
Walstrom, Katherine M.
Warrick, Eric C.
Gasper, Brittany J.
Advisor
Issue Date
2017
Keyword
Prodigiosin
Vibrio
antimicrobial
biosynthesis
prodiginine
Degree
Department
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Abstract

Prodiginines are secondary metabolites produced by several known species of bacteria. These metabolites are known for their bright pigmentation and their potential medicinal uses. Biosynthesis of prodiginine compounds, including the well-studied prodigiosin, has been well characterized in Serratia marcescens and other bacterial species, including several marine bacteria. In an effort to isolate and identify natural products from marine organisms, an environmental sample was taken from a salt flat along the Florida Gulf Coast and cultured for bacterial growth. A bacterial species that produces a vibrant pink pigment was isolated and identified as a member of the Vibrio genus and was named MI-2. Whole genome sequencing identified a 13-gene operon with homology to the S. marcescens prodigiosin biosynthetic operon. The pigment produced by MI-2 was hypothesized to be composed of prodigiosin or related prodiginine compounds and was purified by flash column chromatography and identified by mass spectrometry.