his investigation compared adipose tissue (AT) biomarkers obtained from two biopsy techniques, Bergström and Mercedes. Baseline abdominal subcutaneous AT was obtained from 14 (9M, 5F; 28 ± 4 y; 85 ± 8 kg; Body Fat 28 ± 8%; VO2max 3.3 ± 0.7 L•min-1) healthy, recreationally active adults via the Bergström and Mercedes biopsy techniques. Gene expression was measured in AT (n=8) for inflammatory and adipokine genes of interest (IL1β, IL6, IL8, IL10, TNFα, ADIPOQ, LEPD). Plasma (n=14) and AT (n=11) inflammatory cytokine concentrations were assessed (GM-CSF, IFNγ, IL1β, IL2, IL5, IL6, IL8, IL12p70, IL17A, IL23, TNFα, IL4, IL10, IL13). Average yield for the Bergström biopsy was less than the Mercedes biopsy (261 ± 110 mg vs. 951 ± 601 mg; P<0.05). There was a difference in RNA quality between the Bergström (RIN 7.7 ± 0.7) and Mercedes (RIN 8.1 ± 0.5, P<0.05), with RNA from the Mercedes technique having a higher RIN. There was no difference in expression of inflammatory or adipokine genes in tissue obtained with the Bergström or Mercedes adipose biopsy technique (P>0.05). Of the measurable AT cytokine concentrations (GM-CSF, IFNγ, IL8, IL13, IL17A) there was no difference between Bergström and Mercedes tissue (P>0.05). Several biomarkers were analyzed within the tissue obtained, including RNA quality, select genes of interest, and cytokines. It was shown that both techniques yielded RNA that was acceptable for downstream assays. In the current study there was no difference in the genes of interest or the inflammatory cytokines measured between adipose biopsy techniques at this timepoint. Overall, the Bergström technique yields less tissue but may allow for more individual biopsies to occur compared to the Mercedes technique which yielded more tissue but may limit the number of adipose biopsies. These findings provide valuable insight into the interchangeability of the biopsy techniques based upon study design and desired downstream assays.